METODE EKSTRAKSI DNA CABAI (<em>Capsicum annuum</em> L.) MENGGUNAKAN MODIFIKASI BUFFER CTAB (CETHYL TRIMETHYL AMMONIUM BROMIDE) TANPA NITROGEN CAIR
نویسندگان
چکیده
منابع مشابه
Biopolymer-surfactant Interactions: Binding Studies of Cetyltrimethyl Ammonium Bromide, Cetyl Pyridinium Bromide and Dodecyl Trimethyl Ammonium Bromide with Β-lactoglobulin
Binding studies of cationic detergents such as cetyltrimethyl ammonium bromide, Cetyl pyridinium bromide and dodecyl trimethyl ammonium bromide with β Lactoglobulin were carried out by equilibrium dialysis, ultraviolet difference and circular dichroism techniques at 25 C. Binding isotherms at pH 5·0, 7·0 and 9·0 show cooperative binding at all concentrations of detergents and the number of avai...
متن کاملMyristyl trimethyl ammonium bromide and octadecyl trimethyl ammonium bromide are surface-active small molecule dynamin inhibitors that block endocytosis mediated by dynamin I or dynamin II.
Dynamin is a GTPase enzyme involved in membrane constriction and fission during endocytosis. Phospholipid binding via its pleckstrin homology domain maximally stimulates dynamin activity. We developed a series of surface-active small-molecule inhibitors, such as myristyl trimethyl ammonium bromide (MiTMAB) and octadecyltrimethyl ammonium bromide (OcTMAB), and we now show MiTMAB targets the dyna...
متن کاملThe Germicidal Properties of Certain Quarternary Ammonium Salts With Special Reference to Cetyl-Trimethyl-Ammonium Bromide.
In 1916 Jacobs, Heidelberger, and coworkers at the Rockefeller Institute reported the discovery of a new class of synthetic compounds which possessed potent bactelicidal properties. By reacting hexamethylenetetramine with a large variety of substituted benzylor alkyl-halides, nujmerous quaternary salts of this base were obtained. It was observed that most of these salts had pronounced bacterici...
متن کاملThermodynamic Studies on the Interaction of Histone H2B with Dodecyl Trimethyl Ammonium Bromide
The interaction of histone H2B and dodecyl trimethyl ammonium bromide (DTAB) was studies via equilibrium dialysis method at two different temperatures, at pH 6.4 in phosphate buffer. The binding data were used to obtain the Gibbs free energy of interaction, which is interpreted in terms of a theoretical model based on the Wyman binding potential. The data were then used to obtain...
متن کاملComparison of four methods of extracting DNA from D. gallinae (Acari: Dermanyssidae).
Dermanyssus gallinae is one of the most serious ectoparasites of poultry and it has been implicated as a vector of several major pathogenic diseases. Molecular detection of such pathogens in mites is crucial and therefore, an important step is the extraction of their DNA from mites. So, we compared four DNA extraction protocols from engorged and unfed individual mites: a conventional method usi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Scripta Biologica
سال: 2017
ISSN: 2355-3138
DOI: 10.20884/1.sb.2017.4.2.423